Measurement

Part:BBa_J119401

Designed by: Avery Umphreys   Group: Eckdahl Lab   (2016-07-18)


tCloneTet+PDB riboswitch with P5 and BD2

The BD2 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD2 was inserted via Decoupled Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 83
    Illegal NheI site found at 458
    Illegal NotI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 83
    Illegal BamHI site found at 604
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 83
    Illegal XbaI site found at 98
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 998
    Illegal NgoMIV site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None